1 00:00:03,466 --> 00:00:05,200 This is the Chemical Oceanography Lab, 2 00:00:05,200 --> 00:00:06,333 and I'm going to show you guys around 3 00:00:06,333 --> 00:00:07,633 kind of the different samples 4 00:00:07,633 --> 00:00:08,666 that we take in the field 5 00:00:08,666 --> 00:00:09,600 and how we analyze them 6 00:00:09,600 --> 00:00:10,533 in the lab. 7 00:00:11,033 --> 00:00:12,066 This instrument 8 00:00:12,066 --> 00:00:13,433 is called the 9 00:00:13,433 --> 00:00:14,566 Elemental Analyzer. 10 00:00:14,566 --> 00:00:15,666 We use it to measure 11 00:00:15,666 --> 00:00:17,700 particulate organic carbon. 12 00:00:17,700 --> 00:00:18,433 So in the field, 13 00:00:18,433 --> 00:00:19,933 what we do is we'll filter seawater 14 00:00:19,933 --> 00:00:20,800 to get rid of the water 15 00:00:20,800 --> 00:00:22,100 and keep the stuff that's in it. 16 00:00:22,100 --> 00:00:23,433 And then the filter 17 00:00:23,433 --> 00:00:24,566 goes into this machine 18 00:00:24,566 --> 00:00:25,866 where it will be combusted. 19 00:00:25,866 --> 00:00:27,500 So it'll be burned up in a tube 20 00:00:27,500 --> 00:00:28,033 that is at 21 00:00:28,033 --> 00:00:30,900 950 degrees Celsius. So quite hot. 22 00:00:30,933 --> 00:00:32,133 And then once it's busted up, 23 00:00:32,133 --> 00:00:32,900 all the gas that 24 00:00:32,900 --> 00:00:33,666 releases off of that 25 00:00:33,666 --> 00:00:34,633 gets pushed against 26 00:00:34,633 --> 00:00:35,633 another detector, 27 00:00:35,633 --> 00:00:36,700 and that will measure 28 00:00:36,700 --> 00:00:38,233 carbon and nitrogen. 29 00:00:38,233 --> 00:00:39,100 So this instrument is 30 00:00:39,100 --> 00:00:40,000 how we measure 31 00:00:40,000 --> 00:00:41,466 what's called CDOM. 32 00:00:41,466 --> 00:00:42,833 And it's how we kind of get a look 33 00:00:42,833 --> 00:00:44,300 at what color the water is 34 00:00:44,300 --> 00:00:46,466 if you take all this stuff out. 35 00:00:46,600 --> 00:00:47,300 So this is what typical 36 00:00:47,300 --> 00:00:48,633 seawater looks like. 37 00:00:48,633 --> 00:00:49,366 The higher wavelengths, 38 00:00:49,366 --> 00:00:50,266 you have a little dip here. 39 00:00:50,266 --> 00:00:50,966 That's kind of what 40 00:00:50,966 --> 00:00:51,966 salt looks like. 41 00:00:51,966 --> 00:00:53,333 And then up here in the lower 42 00:00:53,333 --> 00:00:54,933 wavelengths and your ultraviolet, 43 00:00:54,933 --> 00:00:55,666 you have a higher increase 44 00:00:55,666 --> 00:00:56,400 in signal. 45 00:00:56,400 --> 00:00:57,766 And that's where our CDOM is. 46 00:00:57,766 --> 00:00:59,666 So this is the last instrument 47 00:00:59,666 --> 00:01:00,500 that I work with 48 00:01:00,500 --> 00:01:01,366 on a regular basis. 49 00:01:01,366 --> 00:01:02,366 We use it to measure 50 00:01:02,366 --> 00:01:03,900 dissolved organic carbon. 51 00:01:03,900 --> 00:01:04,466 For this one. 52 00:01:04,466 --> 00:01:05,133 We keep the water 53 00:01:05,133 --> 00:01:06,200 that goes through the filter. 54 00:01:06,200 --> 00:01:07,266 So we don't want to know 55 00:01:07,266 --> 00:01:08,066 what's in the particles, 56 00:01:08,066 --> 00:01:08,833 what's in those cells, 57 00:01:08,833 --> 00:01:09,766 we want to know what those cells 58 00:01:09,766 --> 00:01:11,466 are releasing into the environment. 59 00:01:11,733 --> 00:01:12,333 So I'll show you guys 60 00:01:12,333 --> 00:01:13,533 how we keep our glassware 61 00:01:13,533 --> 00:01:13,900 clean. 62 00:01:13,900 --> 00:01:14,633 We have 63 00:01:14,633 --> 00:01:15,400 one, 64 00:01:15,400 --> 00:01:16,300 two and 65 00:01:16,300 --> 00:01:17,233 three different baths 66 00:01:17,233 --> 00:01:18,733 all of our dishes go through. 67 00:01:18,733 --> 00:01:19,566 They spend one day 68 00:01:19,566 --> 00:01:20,400 in each bath. 69 00:01:20,400 --> 00:01:21,300 It's a long process. 70 00:01:21,300 --> 00:01:23,566 To wash one dish is four days of work. 71 00:01:23,600 --> 00:01:24,433 We need to get rid of all of 72 00:01:24,433 --> 00:01:25,766 what we have in this lab 73 00:01:25,766 --> 00:01:26,433 to make sure we're 74 00:01:26,433 --> 00:01:27,533 measuring accurately 75 00:01:27,533 --> 00:01:28,833 what's going on in the ocean. 76 00:01:42,500 --> 00:01:44,100 So what they don't tell you about science 77 00:01:44,100 --> 00:01:47,100 is you're gonna be doing a lot of dishes. 78 00:01:56,733 --> 00:01:57,500 The other thing to note 79 00:01:57,500 --> 00:01:59,800 is they spend one day in each bath, 80 00:01:59,800 --> 00:02:01,766 so they're not it's not a quick half hour 81 00:02:01,766 --> 00:02:02,200 a dunk. 82 00:02:02,200 --> 00:02:03,200 It's overnight 83 00:02:03,200 --> 00:02:05,033 in this bath, overnight in this bath, 84 00:02:05,033 --> 00:02:06,200 overnight in this bath 85 00:02:06,200 --> 00:02:07,933 and usually overnight in here 86 00:02:07,933 --> 00:02:08,700 because I'll start this 87 00:02:08,700 --> 00:02:10,300 at the end of the day. 88 00:02:10,300 --> 00:02:13,300 So 89 00:02:16,866 --> 00:02:17,833 Sometimes it feels like 90 00:02:17,833 --> 00:02:18,966 that's at home day. 91 00:02:18,966 --> 00:02:19,566 Yeah. Right. 92 00:02:19,566 --> 00:02:22,566 It feels like never doing anything for 93 00:02:33,566 --> 00:02:35,766 It's only 2%, but it's really viscous. 94 00:02:35,766 --> 00:02:36,100 Kind of. 95 00:02:36,100 --> 00:02:37,833 Imagine your dish soap at home. 96 00:02:37,833 --> 00:02:39,233 It's about that thick, 97 00:02:39,233 --> 00:02:42,300 this next one is also a bath. 98 00:02:42,300 --> 00:02:43,466 Soap bath. 99 00:02:43,466 --> 00:02:44,400 This is a bleach based 100 00:02:44,400 --> 00:02:46,366 solution called Rb's. 101 00:02:46,366 --> 00:02:47,800 So this is our second bath 102 00:02:47,800 --> 00:02:48,800 that it goes through. 103 00:02:48,800 --> 00:02:50,166 And then this is our third bath. 104 00:02:50,166 --> 00:02:51,633 The third bath is hydrochloric 105 00:02:51,633 --> 00:02:53,266 acid at 10%. 106 00:02:53,266 --> 00:02:55,433 Obviously bleach and acid 107 00:02:55,433 --> 00:02:56,433 do not go well together. 108 00:02:56,433 --> 00:02:57,166 Do not mix those. 109 00:02:57,166 --> 00:02:58,166 So we have to be really careful 110 00:02:58,166 --> 00:02:59,500 about rinsing between. 111 00:02:59,500 --> 00:03:00,233 And when we rinse, 112 00:03:00,233 --> 00:03:01,600 we rinse with milk water. 113 00:03:01,600 --> 00:03:03,866 So this is not standard tap water. 114 00:03:03,866 --> 00:03:04,700 This is water 115 00:03:04,700 --> 00:03:07,133 that's been purified through many filters 116 00:03:07,133 --> 00:03:09,000 and ultraviolet lights 117 00:03:09,000 --> 00:03:11,633 to give it an end goal of 118 00:03:11,633 --> 00:03:14,900 about three parts per billion of carbon. 119 00:03:14,900 --> 00:03:15,866 So we're looking at very, 120 00:03:15,866 --> 00:03:16,966 very low carbon. 121 00:03:16,966 --> 00:03:18,200 If it's glass 122 00:03:18,200 --> 00:03:18,866 it will then get 123 00:03:18,866 --> 00:03:20,200 put into this 124 00:03:20,200 --> 00:03:23,166 instrument is called a combustion oven. 125 00:03:23,166 --> 00:03:24,566 So it goes in here 126 00:03:24,566 --> 00:03:27,533 and then it gets heated to about 490°C 127 00:03:27,533 --> 00:03:28,933 for about four hours. 128 00:03:28,933 --> 00:03:31,933 So that burns off all the carbon 129 00:03:32,033 --> 00:03:33,500 that's also similar temperature 130 00:03:33,500 --> 00:03:35,666 to what our tech machine does. 131 00:03:35,666 --> 00:03:38,400 So really you're burning carbon it. 132 00:03:38,400 --> 00:03:39,766 And we want absolutely no carbon 133 00:03:39,766 --> 00:03:40,966 in our glassware 134 00:03:40,966 --> 00:03:42,333 because we're measuring really, really 135 00:03:42,333 --> 00:03:43,933 tiny amounts of carbon in the ocean. 136 00:03:43,933 --> 00:03:46,933 The open ocean has very little nutrients, 137 00:03:46,933 --> 00:03:49,066 very little carbon, very little nitrogen. 138 00:03:49,066 --> 00:03:49,633 So 139 00:03:58,066 --> 00:04:00,933 Yeah, lots of beds. 140 00:04:00,933 --> 00:04:01,633 Yes. 141 00:04:01,633 --> 00:04:02,500 That's, that's 142 00:04:02,500 --> 00:04:03,700 that's stood out last time. 143 00:04:03,700 --> 00:04:06,166 Yeah. Yeah, it's 144 00:04:06,166 --> 00:04:06,766 it's hard. 145 00:04:06,766 --> 00:04:09,700 We did just get a dishwasher. 146 00:04:09,700 --> 00:04:11,000 We need to get the detergent for it. 147 00:04:11,000 --> 00:04:12,033 And hopefully that automates it 148 00:04:12,033 --> 00:04:13,866 because this is all done by hand, 149 00:04:13,866 --> 00:04:17,666 so I can take me a couple hours a day. 150 00:04:17,966 --> 00:04:20,966 151 00:04:53,400 --> 00:04:55,166 So we take measurements in the field. 152 00:04:55,166 --> 00:04:56,433 As long as the measurements 153 00:04:56,433 --> 00:04:58,566 look good and stable and as expected, 154 00:04:58,566 --> 00:04:59,433 then they get compared 155 00:04:59,433 --> 00:05:00,966 against what pieces. 156 00:05:00,966 --> 00:05:02,433 And as long as those match 157 00:05:02,433 --> 00:05:03,700 then we know that piece 158 00:05:03,700 --> 00:05:04,600 is working properly. 159 00:05:04,600 --> 00:05:05,433 And doing you know what 160 00:05:05,433 --> 00:05:06,466 we sent it up there to do. 161 00:05:06,466 --> 00:05:09,266 So 162 00:05:09,266 --> 00:05:10,866 we've had samples 163 00:05:10,866 --> 00:05:12,366 of a lot of what's called chickadees. 164 00:05:12,366 --> 00:05:14,233 Imean, chickadees meum is known 165 00:05:14,233 --> 00:05:15,866 as the sawdust of the sea. 166 00:05:15,866 --> 00:05:17,033 If you look out and it looks like there's 167 00:05:17,033 --> 00:05:18,033 a bunch of bodies in the water, 168 00:05:18,033 --> 00:05:18,900 that's tricot. 169 00:05:18,900 --> 00:05:19,400 They grow in 170 00:05:19,400 --> 00:05:21,233 these really cool, 171 00:05:21,233 --> 00:05:23,233 like, haystack formations, 172 00:05:23,233 --> 00:05:24,333 but called puffs. 173 00:05:24,333 --> 00:05:26,733 Or they'll grow in these really long, 174 00:05:27,800 --> 00:05:29,600 kind of like flatter bundles. 175 00:05:29,600 --> 00:05:31,166 We call those tufts. So